The sedimentation behaviour of ribonuclease-active and -inactive ribosomes from bacteria
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15 صفحه اولSedimentation coefficients of RNA from 70S and 80S ribosomes.
It has been shown' previously that the corrected sedimentation coefficients (820 W) of the ribosomes from 25 species of fungi and 25 species of bacteria, respectively, range from 76.5 to 84.5 Svedberg units (S) and from 63.8 to 72.1S, and that the difference between the arithmetic means, 81.3 and 68.4S, of these two frequency distributions is significant. Since the ribosomes from other eucaryot...
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Santer, Melvin (Haverford College, Haverford, Pa.), and Josephine R. Smith. Ribonuclease sensitivity of Escherichia coli ribosomes. J. Bacteriol. 92:1099-1110. 1966.-The ribonucleic acid (RNA) contained in 70S ribosomes and in 50S and 30S subunits was hydrolyzed by pancreatic ribonuclease. A 7% amount of the RNA was removed from the 70S particle; at 10(-4)m magnesium concentration, a maximum of...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1965
ISSN: 0006-2936
DOI: 10.1042/bj0960671